Journal: Nature medicine

Article Title: Fibroblast growth factor 19 regulates skeletal muscle mass and ameliorates muscle wasting in mice.

doi: 10.1038/nm.4363

Figure Lengend Snippet: Figure 3 β-Klotho is essential for FGF19-induced muscle hypertrophy. Control and Klb mKO mice (8 weeks old) were treated with 0.1 mg/kg of FGF19 or vehicle for 7 d. (a) Muscle weight in grams (left) or in the percentage of body weight (right) of soleus, tibialis anterior (TA) and gastrocnemius (Gastro.) from mice treated with FGF19 or vehicle. (b) Frequency distribution of cross-sectional soleus-muscle fiber area and mean soleus fiber area. n.s., not significant (P = 0.1). (c) Percentage of slow-twitch (type I) and fast-twitch (type II) oxidative fibers in soleus muscles from control and Klb mKO mice. (d) Effect of FGF19 on type I and type II oxidative fiber area in soleus muscles from control and Klb mKO mice. n.s., not significant. n = 5 per group for control mice and n = 3 per group for Klb mKO mice. Data are mean ± s.e.m. Statistical analysis was done using a two- tailed Student’s t-test. *P < 0.05, **P < 0.01.

Article Snippet: The nitrocellulose membranes were incubated with β-Klotho-biotinylated primary antibody (R&D Systems, BAF-2619, 1:400) for 2 h at room temperature (RT).

Techniques: Control, Muscles, Two Tailed Test